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Title :マダラチョウのメスとモンパノキとの相関に関する研究
Title alternative :Studies on Relationship Between Females of Danaid Butterflies and Messerschmidia argentea
Authors :荻原, 和仁
Authors alternative :Ogihara, Kazuhito
Issue Date :Mar-2001
Abstract :平成10年度~平成12年度科学研究費補助金[基盤研究(C)(2)]研究成果報告書
研究概要:1.モンパノキからマダラチョウのメスの摂食刺激物質の単離-モンパノキの小枝を蒸留水で抽出後、得られた水抽出物をスジグロカバマダラのメスに対する摂食刺激活性を指標にゲルろ過およびシリカゲルカラムクロマトグラフィーで分画し、スジグロカバマダラのメスに対して強い摂食刺激活性を示す塩基性の画分を得た。この塩基性の画分をさらにシリカゲルカラムクロマトグラフィーで分画し、6種のピロリチジンアルカロイド(1-6)を単離した。化合物1-6の構造を分光分析学的常法を用いて、それぞれ3'-acetylindicine(1),indicine(2),3'-acetylindicine N-oxide(3),indicine N-oxide(4),6'-methylindicine(5)および6'-methylindicine N-oxide(6)と決定した。2.マダラチョウのメスに対するピロリチジンアルカロイド(1-6)の摂食刺激活性試験結果-沖縄に棲息する5種のマダラチョウ(スジグロカバマダラ、リュウキュウアサギマダラ、カバマダラ、ツマムラサキマダラおよびアサギマダラ)のメスに対する1-6の摂食刺激活性試験を行った結果、スジグロカバマダラに対して4に、リュウキュウアサギマダラに対して3および4に、ツマムラサキマダラに対して1-4に、アサギマダラに対して3および4に、それぞれ摂食刺激活性が観測された。カバマダラのメスに対してはいずれの化合物でもその活性が観測されなかった。以上の結果から、モンパノキに含まれるマダラチョウのメスに対する摂食刺激物質はマダラチョウの種によって異なることが明らかとなった。また、マダラチョウのオスに対するモンパノキに含まれる pharmacophagous behavior刺激物質を調べ比較した結果、モンパノキに含まれるオスのpharmacophagous behavior刺激物質とメスの摂食刺激物質とは同種間でも異なることが明らかとなった。マダラチョウの雌によって摂取されたピロリチジンアルカロイドの行方については2種のチョウについて明らかになりつつあるが、マダラチョウ全体としての結論を得ることはまだできず、なお現在研究中である。
要約(欧文):Male danaid butterfly of many species is well-known to visit Boraginaceae plants and sucks with its proboscis the pyrrolizidine alkaloids-containing exudation, which was defined as pharmacophagous behavior. The male stores the alkaloids as defensive substances and/or manufactures aphrodisiacal substances from the alkaloids and uses them in its courtship display to attract the female butterfly. The males of danaid butterflies such as Salatura genutia (CRAMER), Ideopsis similis (LINNAEUS), Euploea mulciber (CRAMER), Parantica sita (KOLLAR), and Anosia chrysippus (LINNAEUS) which inhabit in Okinawa also visited Messerschmidia argentea Johnst (Boraginaceae) and sucked dewdrops on the fresh twigs and their own saliva on the dead twigs of the plant. In some species, the same pharmacophagous behavior is observed for not only the male butterflies but also the female ones. I examined the differential effects of constituents from M.argentea on pharmacophagous behavior of the male and female danaid butterflies. A water extract of the twigs of M.argentea was subjected to gel filtration chromatography on Sephadex G-25 and then column chromatography on silica gel to give a basic fraction which stimulated ingestion of it by the male and female butterflies. The fraction was, moreover, subjected to preparative TLC to give four pyrrolizidine alkaloids (PAs) such as 3'-acetylindicine (1), indicine (2), 3'-acetylindicine-N-oxide (3), indicine-N-oxide (4), 6'-methylindicine (5), and 6'- methylindicine-N-oxide (6). Phagostimulant tests of PAs 1-6 against the adult males and females of danaid butterflies were done. The male butterflies of S.genutia ingested 1-4. The female ones ingested only 4, but did a sample mixed 4 such as 1 + 2 + 3 + 4 + 5 + 6. Thus, each of the alkaloid 1-4 was a stimulant of the pharmacophagous behavior into the males of S.genutia and 4 into the females. On the basis of phagostimulant test for other butterflies, the alkaloid 3 was found to be a stimulant of the pharmacophagous behavior into the males of I.similis, 3 and 4 into of the females of I.similis, . Each of the alkaloid 1-4 was a stimulant of the pharmacophagous behavior into the males and females of E.mulciber. Each of the alkaloid 1-4 was a stimulant of the pharmacophagous behavior into the males of P.sita and 3 and 4 into females of P.sita. he alkaloid 4 was a stimulant of the pharmacophagous behavior into of the males of A.chrysippus. The female butterflies of A.chrysippus was not stimulated by the water extract and each pyrrolizidine alkaloid.
Type Local :研究報告書
Publisher :荻原和仁
URI :http://hdl.handle.net/20.500.12000/14313
Appears in Collections:Research Report (Faculty of Science)

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