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Title :大腸発癌過程におけるNotch シグナル経路による分化制御機構の分子病理学的検討
Title alternative :Investigation of the molecular pathology of the differentiation mechanism by the Notch signaling pathway in colon carcinogenesis process.
Authors :森岡, 孝満
吉見, 直己
Authors alternative :Morioka, Takamitsu
Yoshimi, Naoki
Issue Date :1-Jun-2010
Abstract :2007年度~2009年度科学研究費補助金(基盤研究(C))研究成果報告書
研究概要(和文):ラット大腸発癌モデルで作製した腫瘍を用いてNotch1とKlf4の発現をRT-PCR にて調べた結果、Notch1の発現亢進と杯細胞の分化に関与しているKlf4の発現低下がラット大腸発癌過程に関与していることが示唆された。57症例のヒト大腸粘膜を用いて粘液枯渇巣(MDF)の検出を試みた結果、ヒト大腸粘膜においてもラット大腸発癌モデル同様にMDFの存在が明らかとなった。ヒトMDFは、組織学的に杯細胞の減少、核の腫大、核の重層化及び間質へのリンパ球浸潤を認めた。また、幾つかのOverlapped lesion(OLL)は、鋸歯状変化が認められた。以上よりMDFがヒトにおいても大腸癌の前癌病変として有用なバイオマーカーに成り得る可能性が考えられた。
研究概要(英文) :The aim of this study is to investigate the relation of Notch signaling pathway on colon carcinogenesis by using the rats colon carcinogenic model. We found that the expression of Notch1 in colon tumor was significantly increased compared to the normal mucosa. In contrast, the expression of Klf4 in colon tumor was significantly decreased compared the normal mucosa. These results suggested that increased expression of Notch1 and decreased expression of Klf4 could be associated with the rat colon carcinogenesis. We also examined the identification of mucin-depleted foci (MDF) by using alcian blue pH2.5 stain in human colon mucosa of 57 subjects. We found that MDF existed in human colon mucosa. Histologically, MDF and OLL (Overlapped lesion with ACF) recognized the loss of goblet cells, nucleus swelling, nuclear stratification, and infiltrate of lymphocyte to stroma. The some of OLL represented the crypt with serrated change. These results suggested that MDF existed even in humans and it may be preneoplastic lesions and could be useful biomarker of human colon carcinogenesis.
Type Local :研究報告書
Publisher :森岡孝満
URI :http://hdl.handle.net/20.500.12000/17847
Appears in Collections:Research Report (Faculty of Medicine)

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