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The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus >
No.47 (2000/12) >

Title :第2報 緩照射の場合(ガンマ線照射によるセイロンベンケイソウの CAM/C_3 型光合成突然変異株の作出)
Title alternative :In case of a acute irradiation (On the production of photosynthesis mutant in Kalanchoe pinnata using the gamma-ray irradiation)
Authors :川満, 芳信
渡慶次, 努
川元, 恵子
永冨, 成紀
野瀬, 昭博
村山, 盛一
Authors alternative :Kawamitsu, Yoshinobu
Tokeshi, Tsutomu
Kawamoto, Keiko
Nagatomi, Shigeki
Nose, Akihiro
Murayama, Seiichi
Issue Date :1-Dec-2000
Abstract :本実験では, CAM植物の光合成効率を高めるためにC_3型光合成への変更の可能性を培養段階での昼夜温度の逆転から検討し, 更に, ガンマ線緩照射による変異個体の作出について検討した。得られた結果の概要は以下の通りである。1. セイロンベンケイソウの培養植物は, 明期において活発なCO_2吸収を示すC_3型光合成的ガス代謝を行いながらも, CAM植物特有のリンゴ酸の日変化が見られCAM cycling型光合成を行った。2. 培養段階においても高夜温条件によりリンゴ酸蓄積量が減少し, C_3型光合成に近づいた。3. カルス分化時から高夜温で生育させた培養植物体は光合成型を変更したが, 順化させると本来のCAM型光合成が回復した。4. 昼夜のリンゴ酸含量の差(△リンゴ酸)におけるガンマ線照射の効果についてはほとんど影響がなかった。5. ガンマ線照射個体は気孔密度において増加傾向を示し, 孔辺細胞長においては影響がなかった。また, 培養を経由した植物体において気孔密度は減少傾向を示し, 孔辺細胞長は肥大する傾向を示した。
In this paper, the possibility of the change to the C_3 type photosynthesis was examined from the inversion of the daytime and nighttime temperature in the culture stage in order to raise photosynthesis efficiency of a CAM plant (Ceylon Benkei, Kalanchoe pinnata), and in addition, the production of the mutation by gamma-ray chronic irradiation was examined. Outlines of the result obtained are as follows; 1. Culture plants of the Ceylon Bennkei performed CAM cycling which showed the daily variation of malic acid which is peculiar for a CAM plant, while C_3 type gas exchange which shows the active CO_2 absorption in the light period was done. 2. The malic acid accumulation decreased by the high night temperature condition in the culture stage, and it approached the C_3 type photosynthesis. 3. Cultured plants which were made to grow at the high night temperature from the callus differentiation made the photosynthesis type change, and original CAM type photosynthesis recovered, when the plants acclimatized. 4. There was seldom the effect of the gamma-ray irradiation for the difference (Δ malic acid) in malic acid content between daytime and nighttime. 5. Stomatal frequency of the gamma-ray irradiating plants showed the increase tendency, and it was not effective at guard cell length. In addition, stomatal frequency showed the decline in the plants which went through the culture, and guard cell length showed hypertrophying tendency.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/3414
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.47 p.17 -28
Appears in Collections:No.47 (2000/12)

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