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No.43 (1996/12) >

Title :乳用牛直腸便由来大腸菌の薬剤耐性(生物生産学科)
Title alternative :Drug resistance of Escherichia coli strains isolated from the rectal content of dairy cattle (Department of Bioproduction)
Authors :日越, 博信
東江, 靖典
平川, 守彦
Authors alternative :Higoshi, Hironobu
Agarie, Yasunori
Hirakawa, Morihiko
Issue Date :1-Dec-1996
Abstract :沖縄県内の3酪農家で搾乳中の健康な乳用牛(ホルスタイン種),合計78頭の直腸便から分離した大腸菌652株について,7薬剤に対する感受性試験を行い,また耐性菌については伝達性Rプラスミドの検索も試みた。供試した7薬剤のいずれか1薬剤に耐性の大腸菌は,全体では68株(10.4%)であった。酪農家別の耐性菌検出率では,C酪農家5.5%,A酪農家8.7%,B酪農家16.5%であった。薬剤別では,CTC耐性菌が9.5%で最も高く,他の薬剤に対する耐性菌は5%未満であったが,NA耐性菌は検出されなかった。3酪農家由来ともCTC耐性菌が最高であったが,B酪農家由来ではSMおよびSA耐性菌も若干高い値を示した。耐性型の種類は単剤型から6剤型まで10種類認められたが,4剤型は検出されなかった。また全体では,2剤以上の薬剤に耐性の多剤耐性型が過半数を占め,特にB酪農家由来では60%以上が多剤耐性型であり,6剤耐性型も本酪農家由来であった。一方,A及びC酪農家由来では60%以上が逆に単剤耐性型であった。耐性菌68株について,伝達性Rプラスミドの検索を行った結果,A酪農家由来の5株のみがRプラスミドを保有していた。この5株の伝達耐性型は,すべてCTC単剤型であった。
The sensitivity to seven antibiotics of 652 Escherichia coli strains isolated from the rectal contents of 78 healthy dairy cows (Holstein) from three dairy farms in Okinawa Prefecture was tested. Transmissible R plasmid was also examined in resistant strains. Sixty-eight E. coli strains (10.4%) were resistant to at least 1 of the 7 tested antibiotics. The resistant strain detection rate was 5.5% in Farm C, 8.7% in Farm A and 16.5% in Farm B. CTC resistance was the most common at 9.5%, resistance to the other antibiotics was less than 5% and NA resistance was not detected. CTC-resistant strains were the most common in all three farms. The proportions of SM-and SA-resistant strains were slightly higher in Farm B. There were 10 resistance patterns ranging from 1-agent to 6-agent resistance. Over half (51.5%) of all the resistant strains were resistant to 2 or more agents, particularly, over 60% of those from Farm B were multi-drug resistant. The six-agent resistant strain was derived from Farm B. In contrast, over 60% of the resistant strains from Farms A and C were resistant to one agent. Transmissible R plasmid was examined in 68 antibiotic resistant E. coli strains. R plasmid was detected only in 5 strains from Farm A, and in all 5 strains the transmissible resistance type was 1-agent type to CTC.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/3723
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.43 p.7 -12
Appears in Collections:No.43 (1996/12)

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