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Title :西太平洋域で発見されたタカサゴとニセタカサゴの雑種
Title alternative :Natural hybridization between Pterocaesio digramma and P. marri collected from Western Pacific
Authors :賀数, 大吾
佐久本, 孟寿
立原, 一憲
今井, 秀行
Authors alternative :Kakazu, Daigo
Sakumoto, Takehisa
Tachihara, Katsunori
Imai, Hideyuki
Issue Date :20-Dec-2015
Abstract :In May 2015, a presumed hybrid fusilier fish of the genus Pterocaesio was collected from Ie-jima Island, Ryukyu Archipelago, Japan. It was morphologically similar to both Pterocaesio digramma and P. marri, but the species could not be clearly identified. To determine if it was a hybrid, we developed a PCR-RFLP marker that could distinguish these two species. After amplifying ITS-1 region with PCR, the PCR product was digested by restriction enzyme Taq Ⅰ. The result showed that the specific bands to each species resulting in heterozygosity, which suggested this individual was a hybrid. This is the first detailed record of natural hybridization among Caesionidae species in Ryukyu Archipelago. Furthermore, we investigated 626 specimens, including adults and juveniles of these two species using the genetic marker we developed in this study. Eighteen hybrid individuals were detected in several localities (Amami-oshima Island, Ie-jima Island, Yaeyama Islands (Ishigaki-jima Island and Iriomote-jima Island), Philippines and Indonesia). Combining nuclear DNA and mtDNA data, two individuals derived from backcross were also detected.
Type Local :雑誌掲載論文
ISSN :0067-8716
Publisher :日本生物地理学会
URI :http://hdl.handle.net/20.500.12000/37567
Citation :日本生物地理学会会報 = Bulletin of the Bio-geographical Society of Japan Vol.70 p.55 -63
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Science)

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