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Title :ミナミメダカ琉球型個体群における他個体群の放流による遺伝的撹乱の初事例
Title alternative :First evidence from genetic markers for introgression of non-native genes into unique Ryukyu subpopulation of Oryzias latipes
Authors :今井, 秀行
米沢, 俊彦
立原, 一憲
Authors alternative :Imai, Hideyuki
Yonezawa, Toshihiko
Tachihara, Katsunori
Issue Date :20-Jan-2017
Abstract :The current study examined the distribution of minami-medaka (Oryzias latipes) across the Ryukyu Archipelago in Japan to assess if the Ryukyu subpopulation as described by Sakaizumi et al. (1983) may be significantly divergent. Between 2003 and 2010, O. latipes were sampled from across Japan and populations compared using two genetic markers, an allozyme locus (aspartate aminotransferase; Aat) originally screened by Sakaizumi et al. (1983) and a new PCR-RFLP mtDNA marker based on the ND1 region. Laboratory trials showed that the Ryukyu subpopulation of O. latipes crossed with the Eastern subpopulation produced viable hybrids possessing both alleles Aatb and Aatc confirming that this marker will be effective for distinguishing natural hybrids between the Ryukyu subpopulation and other subpopulations. Populations distributed from the Amamioshima Islands to the Okinawa Islands all conformed to Ryukyu subpopulation genotypes and were fixed for allele Aatc. In addition, Okinawa islands populations were discriminated for the first time successfully from other subpopulations based on haplotype 5 (PCR-RFLP marker). A population at Chinen on Okinawajima Island possessed different genotypes to other Ryukyu subpopulations suggesting that it may have resulted from an introduction from a non-native source. Genetic introgression from other non-native subpopulations of O. latipes is the first case found in the Ryukyu Archipelago. It will be important in the future therefore, to apply the genetic markers used here to periodically monitor the Ryukyu subpopulation to conserve unique regional genetic diversity.
Type Local :雑誌掲載論文
ISSN :0067-8716
Publisher :日本生物地理学会
URI :http://hdl.handle.net/20.500.12000/37568
Citation :日本生物地理学会会報 = Bulletin of the Bio-geographical Society of Japan Vol.71 p.121 -129
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Science)

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