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Title :Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific
Authors :Mino, Sayaka
Makita, Hiroko
Toki, Tomohiro
Miyazaki, Junichi
Kato, Shingo
Watanabe, Hiromi
Imachi, Hiroyuki
Watsuji, Tomo-o
Nunoura, Takuro
Kojima, Shigeaki
Sawabe, Tomoo
Takai, Ken
Nakagawa, Satoshi
Issue Date :28-Feb-2013
Abstract :Deep-sea hydrothermal vent fields are areas on the seafloor with high biological productivity fueled by microbial chemosynthesis. Members of the Aquificales genus Persephone/la are obligately chemosynthetic bacteria, and appear to be key players in carbon, sulfur, and nitrogen cycles in high temperature habitats at deep-sea vents. Although this group of bacteria has cosmopolitan distribution in deep-sea hydrothermal ecosystem around the world, little is known about their population structure such as intraspecific genomic diversity, distribution pattern, and phenotypic diversity. We developed the multi-locus sequence analysis (M LSA) scheme for their genomic characterization. Sequence variation was determined in five housekeeping genes and one functional gene of 36 Persephone/la hydrogeniphila strains originated from the Okinawa Trough and the South Mariana Trough (SNT). Although the strains share >98.7% similarities in 16S rRNA gene sequences, MLSA revealed 35 different sequence types (ST), indicating their extensive genomic diversity. A phylogenetic tree inferred from all concatenated gene sequences revealed the clustering of isolates according to the geographic origin. In addition, the phenotypic clustering pattern inferred from whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis can be correlated to their MLSA clustering pattern. This study represents the first MLSA combined with phenotypic analysis indicative of allopatric speciation of deep-sea hydrothermal vent bacteria.
URL :https://doi.org/10.3389/fmicb.2013.00107
Type Local :雑誌掲載論文
ISSN :1664-302X
Publisher :Frontiers Media
URI :http://hdl.handle.net/20.500.12000/37687
Citation :Frontiers in microbiology Vol.4 no.107
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Science)

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