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No.40 (1993/12) >

Title :豚枝肉及び枝肉解体器具類由来大腸菌群の薬剤耐性(生物生産学科)
Title alternative :Drug resistance of Coliform bacteria isolated from dressed pork carcasses, and machines and equipment for pork processing (Department of Bioproduction)
Authors :日越, 博信
上原, 亮
内田, 雅美
Authors alternative :Higoshi, Hironobu
Uehara, Makoto
Uchida, Masami
Issue Date :1-Dec-1993
Abstract :食肉における薬剤耐性菌の汚染状況を知る目的で,と畜場内の豚枝肉及び枝肉解体器具類から大腸菌群を分離し,ABPC, CTC, SM, CP, KM, NA, SAの7薬剤に対する感受性試験を行った。豚枝肉由来のE.coliでは,18株中15株(83.3%)が耐性であったが,解体器具類由来では17株全てが感受性であった。一方E.coli以外の菌株(C.freundii及びK.pneumoniae)では,豚枝肉由来の57株中25株(43.3%)が,また解体器具類由来の133株中36株(27.1%)が,それぞれ耐性であった。薬剤別の耐性菌は,E.coliではCTC及びSA耐性が各86.7%で最も高く,E.coli以外の大腸菌群では豚枝肉及び解体器具類由来でABPC耐性が,それぞれ36.0%と72.2%で最も高かった。耐性型は,E.coliでは単剤耐性型から6剤耐性型まで7種類あったが,2剤耐性型が最多であった。E.coli以外の大腸菌群の耐性型は,豚枝肉由来の全てが単剤型で4種類,解体器具類由来では単剤型から4剤耐性型まで8種類あったが,両者ともABPC単剤型が最も多かった。Rプラスミド保有菌は,豚枝肉由来のE.coli及びK.pneumoniaeから1株ずつ検出され,いずれも単剤伝達型であった。
To assess the contamination of meat by drug-resistant bacteria, coliform bacteria were isolated from dressed pork carcasses, and from pork processing machines and equipment in a slaughterhouse. These bacteria were tested for sensitivity to ABPC, CTC, SM, CP, KM, NA and SA. Eighteen strains of Escherichia coli were isolated from pork carcasses. Fifteen of these 18 strains (83.3%) were drug-resistant. The samples were taken from 6 carcasses prior to washing, 6 carcasses prior to grading, and 6 carcasses in refrigerated storage. Seventeen strains were found on machines and equipment used for meat processing (table, knife, skinner, and cotton gloves). All 17 strains were sensitive to drugs. There were 57 strains other than E. coli found on the carcasses (such as Citro-bacter freundii and Klebsiella pneumoniae). Twenty five of these 57 (43.9%) exhibited drug resistance. There were 133 different strains found on the machines and equipment, of which 36 (27.1%) were drug-resistant. The E. coli revealed the most frequent resistance to CTC and SA (86.7%, respectively). The C. freundii and K. pneumoniae isolated from pork carcasses and the machines and equipment revealed the highest resistance to ABPC (36.0% and 72.2%, respectively). A review of the patterns of drug resistance revealed that E. coli exhibited seven patterns of drug resistance ranging from single drug to 6-drug resistance. Resistance to two-drug was the most common finding. C. freundii and K. pneumoniae found on the carcasses revealed four different types of single drug resistance. Examples of these two strains found on the machines and equipment exhibited eight patterns of resistance varying from single to 4-drug resistance. ABPC resistance was most frequent in both of these groups. One strain each of E. coli and K. pneumoniae isolated from pork carcasses was found to retain R plasmid. Each showed single drug resistance.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/3789
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.40 p.29 -34
Appears in Collections:No.40 (1993/12)

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