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No.39 (1992/12) >

Title :サトウキビ葉・梢頭部の化学的前処理と酵素糖化性(生物資源科学科)
Title alternative :Chemical pretreatment and enzymatic saccharification of sugarcane top & leaves (Department of Bioscience and Biotechnology)
Authors :石原, 昌信
有満, 和人
当山, 清善
Authors alternative :Ishihara, Masanobu
Arimitsu, Kazuto
Toyama, Seizen
Issue Date :1-Dec-1992
Abstract :サトウキビ葉・梢頭部を酵素糖化したのち糖化液を発酵原料に供することを目的として,サトウキビ葉・梢頭部の化学的前処理と酵素糖化性等について調べた。サトウキビ葉・梢頭部を5%NaClO,5%NaClO_2,35%H_2O_2 : CH_3COOH(1 : 1)及び0.5%NaOH溶液を用いて120℃で20分間前処理を行なうと酵素分解性が著しく高められた。また,これら化学薬品処理は原料の34∿60%に相当する成分を可溶化した。酵素分解性からみた最適アルカリ濃度は0.5∿1.0%であり,本NaOH処理基質はセルラーゼを用いた48時間の酵素作用でほぼ完全に糖化された。アルカリ処理に伴う重量減少量はアルカリ濃度に依存して増加した。0.5%NaOH処理葉・梢頭部の酵素糖化性は処理温度の上昇に伴ない増大した。また同基質の酵素化率は添加量の増加に伴ない急激に減少し,10%の基質濃度では約60%程度であった。
The effectiveness of various chemical pretreatments of sugarcane top & leaves on enzymatic hydrolysis of fibrous components was evaluated. The treatment with 5% sodium perchlorate, 5% sodium chlorate, 35% peroxide : acetate (1 : 1) and 0.5% sodium hydroxide significantly increased the degradability and resulted in loss of weight in the range from 34 to 60% of original material. Pretreatments with 0.5 to 1.0% sodium hydroxide found to be effective for increasing its susceptibility to enzymatic hydrolysis. The alkali-treated materials were almost completely degraded by cellulase preparation in 48 hr of incubation. The degree of weight loss by pretreatment with sodium hydroxide was dependent on the concentration of reagent. Susceptibility of 0.5% sodium hyroxide-treated materials to hydrolysis action of the enzyme increased in proportion to the rise in temperature of pretreatment. The rate of saccharification of 0.5% sodium hydroxide-treated materials by the enzyme decreased with an increase in concentration of substrate.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/3815
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.39 p.109 -113
Appears in Collections:No.39 (1992/12)

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