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No.36 (1989/12) >

 
Title :バガスの酵素分解性とフェノール化合物(農芸化学科)
Title alternative :Enzymatic degradation of sugar-cane bagasse and phenolic compounds (Department of Agricultural Chemistry)
Authors :石原, 昌信
石垣, 勝己
当山, 清善
Authors alternative :Ishihara, Masanobu
Ishigaki, Katsumi
Toyama, Seizen
Issue Date :5-Dec-1989
Abstract :粉砕バガスの酵素分解反応を行ない,酵素分解生成物の紫外部吸収値とフェノール化合物について調べた。酵素分解生成物をNaOH溶液で前処理すると280nm吸収値が著しく増加した。NaOH処理した酵素分解生成物にはフェルラ酸以外のフェノール化合物は認められなかった。酵素分解生成物中のフェルラ酸をペーパークロマトグラフィーにより他成分と分離するには試料をNaOHで前処理する必要があった。バガス中のフェノール化合物量とバガスの酵素分解性との間には相関性が認められた。酵素分解生成物はゲル濾過により高分子及び低分子画分に分画され,両画分にはフェルラ酸と糖が存在した。高分子画分の糖成分はグルコース,キシロース及びアラビノースであった。
The ultraviolet absorbance of the supernatant and the water soluble phenolic compounds released were determined after treating sugar-cane bagasse with a commercial cellulase preparation,Meicelase. When the supernatant was treated with NaOH solution at 120℃ for 30min, the absorption value at 280nm was increased. Ferulic acid, the only phenolic compound detected, was extracted with ethylacetate from the supernatant which was treated with NaOH. Ferulic acid which was released from bagasse during enzyme reaction could be separated from the other substances by paperchromatography. when sugar-cane bagasse was treated with NaOH, the degradability of the residues by cellulase preparation was strongly correlated with the amount of phenolic compound released by the alkali. Gel filtration of the enzyme hydrolysate on Toyopear HW 40 gave low-and high-molecular-weight fractions, both of which appeared to contain phenolic-complexes. The high-molicular-weight fraction obtained by gel filtration contained glucose, xylose and arabinose.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/3876
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.36 p.59 -65
Appears in Collections:No.36 (1989/12)

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