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No.35 (1988/12) >

 
Title :Bacillus sp. No. 430 のα-L-アラビノフラノシダーゼの精製と性質(農芸化学科)
Title alternative :Purification and Properties of α-L-Arabinofuranosidase II from Bacillus sp. No. 430 (Department of Agricultural Chemistry)
Authors :安田, 正昭
徳里, 政丈
Authors alternative :Yasuda, Masaaki
Tokuzato, Masatake
Issue Date :5-Dec-1988
Abstract :Bacillus sp. No. 430の生産するアラビナン分解酵素の精製を行い, その酵素化学的性質を検討した。酵素の精製は, 供試菌株の培養濾液から硫酸アンモニウム分画, DEAE-セルロース, 2回めのDEAE-セルロース, QAE-セファデックスA-50,DEAE-トーヨーパール650S及びセファデックスG-75などのカラムクロマトグラフィーを組み合わせて行なった。精製酵素はディスク電気泳動的に高度に精製されていた。精製酵素の反応至適pHは7.0,反応至適温度は40℃であった。本酵素反応はCu^<2+>, Cd^<2+>, α, α'-ジピリジル, 沃度酢酸, パラクロロマーキュリーベンゾエートにより部分的に阻害を受けた。精製酵素はビートアラビナン, アラビノガラクタンやパラーニトロフェニルアラビノフラノシドに作用した。反応生成物はL-アラビノースのみが検出された。本酵素はα-L-アラビノフラノシダーゼとして特徴づけられた。本酵素を用いることにより, さとうきびバガスから高純度のキシランが得られた。
This study was carried out for an effective utilization of agricultural wastes by microbial enzymes. In order to liberate arabinose from arabinose-containing polysaccharides such as arabinan, arabinoxylan, and arabinogalactan, α-L-arabinofuranosidase was investigated. An α-L-arabinofuranosidase II was purified from the culture fluid of Bacillus sp. No. 430,which was isolated from the soil of sugar cane fields. The process of purification was as follows; salting out by ammonium sulfate, first DEAE-cellulose, second DEAE-cellulose, QAE-Sephadex A-50,DEAE-Toyopearl 650 S, and Sephadex G-75 column chromatography. The enzyme was highly purified by the method of disc gel electrophoresis. The purified enzyme had the maximum reactivity at pH 7.0 and 40℃. The enzyme was partially inhibited by Cu^<2+>, Cd^<2+>, α, α′-dipyridyl, iodoacetic acid, and p-chloromercuribenzoic acid. The highly purified enzyme was confirmed to liberate L-arabinose from beet arabinan, arabinogalactan, arabinoxylan, and p-nitrophenyl α-L-arabinofuranoside. The reaction product was paper chromatographically demonstrated to be L-arabinose only. The purified enzyme was inactive for p-nitrophenyl α-, β-D-galactopyranosides and p-nitrophenyl β-D-xylopyranoside. And thus, it was characterized as α-L-arabinofuranosidase which was exo-arabinanase. High purity of xylan from sugar cane bagasse or galactans from coffee bean and soybean was obtained with the enzyme.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/3891
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.35 p.53 -62
Appears in Collections:No.35 (1988/12)

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