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No.33 (1986/12) >

 
Title :クロレラ菌体抽出液中のパパインインヒビターとその性質(農芸化学科)
Title alternative :Presence and partial characterization of papain inhibitor in the extracts of Chlorella cells (Department of Agricultural Chemistry)
Authors :当山, 清善
石原, 昌信
松元, 靖
与那覇, 和雄
Authors alternative :Toyama, Seizen
Ishihara, Masanobu
Matsumoto, Yasushi
Yonaha, Kazuo
Issue Date :5-Dec-1986
Abstract :酢酸含有培地を用い屋外培養池で培養された収穫直後の生クロレラ菌体及び噴霧乾燥粉末クロレラ菌体の抽出液中にパパインインヒビター活性が認められた。本インヒビターはクロレラ菌体を煮沸処理することによって抽出された。本インヒビターはpH2∿9の範囲で98℃, 20分間処理しても安定で, 非透析性の蛋白様物質であった。本インヒビターはパパインとともにキモパパイン及びフィシンに対して阻害効果を示したが, トリプシン及びα-キモトリプシンに対する阻害効果は認められなかった。
The presence and partial characterization of a papain inhibitor of the extracts of Chlorella cells has been investigated. The inhibitory activity was detected in the boiled extracts of freshly harvested Chlorella cells and spraydried powder Chlorella cells which were cultured in the acetic acid-containing medium by using large-scale pond culture plant. The inhibitor was non-dialyzable and seemed to be protein-like substance. The inhibitor was found to be heat-stable (98℃, 20min) at pHs ranging from pH 2 to 9. The inhibitor also inhibited chymopapain and ficin, but it did not have inhibitory activity against trypsin and α-chymotrypsin.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/3924
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.33 p.103 -109
Appears in Collections:No.33 (1986/12)

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