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No.31 (1984/11) >

Title :甘蔗茎の NPP- ホスファターゼの性質(農芸化学科)
Title alternative :Properties of NPP-phosphatase from cane stalks (Department of Agricultural Chemistry)
Authors :仲宗根, 洋子
Authors alternative :Nakasone, Yoko
Issue Date :19-Nov-1984
Abstract :登熟甘蔗茎には, パラニトロフェニルリン酸を加水分解する酸性ホスファターゼが存在することを確認した。蔗汁より, 硫安塩析したのちに酸処理した酵素タンパク画分を, ホスファターゼ酵素標品として用いその性質をしらべた。本酵素は至適pHを5.5にもつ酸性ホスファターゼであった。他の植物起源の酸性ホスファターゼとことなって, 本酵素は1mMのEDTAやカルシウムイオンによりほとんど活性を失なった。本酵素は1mMの水銀イオンおよびフッ素イオンの阻害をうけたが, 銅イオンによっては全く阻害されなかった。また, 至適反応温度は50℃にあって, 10分間保持の40℃までは安定であった。
The existence of an acid phosphatase in the mature cane stalks of NCO 376 variety, cultivated in Okinawa, was confirmed. The acid phosphatase hydrolyzing p-nitrophenyl phosphoric acid (NPP), from the extract of cane stalks, was partially purified by ammonium sulfate fractionation and acid treatment. This enzyme had an optimum pH of 5.5 and almost no activity at alkaline areas. It had Km value for NPP of 0.77mM and an optimum temperature of 50℃ and was stable at temperatures lower than 40℃. Magnesium, cupric and manganese ions in 1.16mM showed no effect on the enzyme activity. However, the inhibition of the activity by calcium and mercuric ions and EDTA in 1.16mM was remarkable, but that by fluoride ion in 1.16mM was moderate. EDTA and mercuric ion in 0.11mM, on the other hand, did not inactivate the enzyme. The modes of the inhibition by EDTA and calcium ion distinguish the enzyme studied in this work from the acid phosphatases obtained from several other plant species.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/3963
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.31 p.51 -55
Appears in Collections:No.31 (1984/11)

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