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The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus >
No.27 (1980/11) >

 
Title :土壌から分離された放線菌によるテンサイ粕アラビナンの分解について(農芸化学科)
Title alternative :On the decomposition of arabinan of beet-pulp by Streptomyces sp. isolated from soils (Department of Agricultural Chemistry)
Authors :安田, 正昭
宮里, 興信
菊池, 修二
Authors alternative :Yasuda, Masaaki
Miyazato, Koshin
Kikuchi, Shuji
Issue Date :29-Nov-1980
Abstract :放線菌のアラビナン分解活性を調べた結果, さとうきび畑から分離したStreptomyces sp.No.44菌株が最も高い活性を有することがわかった。Streptomyces sp.No.44菌株をテンサイ粕抽出液を含むペプトン培地(初発pH6.5∿7.0)で30℃, 36時間振とう培養を行うことによりアラビナナーゼがよく生産された。本酵素はアラビナンを加水分解し, 非還元性末端よりアラビノースのみを生成するいわゆる糖化型アラビナナーゼであることが明らかになった。本酵素の反応最適pHは5.5∿7.0で, 反応最適温度は50℃であった。
The production and some properties of the enzyme hydrolyzing arabinan by Streptomyces sp. were described. The production of the enzyme was investigated with 262 strains of the actinomycetes isolated from soils of sugar-cane fields in Okinawa. The highest production of the enzyme was found in the culture fluid of Streptomyces sp. No. 44. In order to find the medium conditions required for the improvement of the enzyme production by this strain, the organism was grown in the medium containing beet-pulp extract. The best enzyme production was obtained when the organism was grown in the medium (initial pH 6.5-7.0) containing beet pulp extract at 30℃ for 36hr under aerobic conditions. When L-arabinan was hydrolyzed with arabinanase, oligo saccharide was not liberated, but only L-arabinose was liberated. The enzyme had the maximum reactivity at about pH 5.5-7.0 and 50℃ for the arabinanase reaction.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/4091
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.27 p.109 -117
Appears in Collections:No.27 (1980/11)

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