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No.27 (1980/11) >

 
Title :土壌から分離された放線菌による甘蔗バガスヘミセルロースの分解について(農芸化学科)
Title alternative :On the decomposition of hemicellulose of sugar-cane bagasse by Streptomyces sp. isolated from soils (Department of Agricultural Chemistry)
Authors :安田, 正昭
宮里, 興信
島袋, 政朋
Authors alternative :Yasuda, Masaaki
Miyazato, Koshin
Shimabukuro, Masatomo
Issue Date :29-Nov-1980
Abstract :放線菌のヘミセルラーゼ活性を調べた結果, さとうきび畑土壌から分離されたStreptomyces sp.No.131.菌株が最も高い活性を有することがわかった。Streptomyces sp.No.131菌株を甘蔗バガスのヘミセルロース(2%)を含むペプトン培地(初発pH8.5), 30℃, 72hr振とう培養を行うことによりヘミセルラーゼがよく生産された。本酵素は培地中の甘蔗バガスヘミセルロースにより誘導生成された。本菌の生産するヘミセルラーゼは, 甘蔗バガスヘミセルロースを加水分解しキシロオリゴ糖とキシロースを生成する酵素で, 液化型キシラナーゼである。本酵素の反応最適pHは7.5で, 反応最適温度は50℃であった。
The association constants and molar extinction coefficients of the product of the addition reaction of all-trans-retinal and 20 amino acids were determined. Those having the higher pK values of amino group, such as L-ornithine, L-lysine, and β-alanine, gave greater association constants, indicating not only the association constant of retinylidene-compound formation but also the pK value of the amino group involved in the reaction would be utilized as an indicator for the purpose of screening amino-acid related, food-originated insect-vision inhibitors at a molecular level. For aniline and hydroxylamine, the association constants and molar extinction coefficients of the respective product formed were also determined. L-Lysine, aniline, and hydroxylamine modified the photopotentials generated across all-trans-retinal bilayer lipid membranes in appropriate conditions, showing the applicability of the membrane system for screening the insect-vision inhibitors having amino groups.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/4092
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.27 p.119 -128
Appears in Collections:No.27 (1980/11)

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