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No.27 (1980/11) >

 
Title :レサズリンテストを中心とする生乳の細菌学的検査 III(畜産学科)
Title alternative :Bacteriological examination of raw milk with particular reference to resazurin test III (Department of Animal Husbandry)
Authors :日越, 博信
森山, 高広
諸見, 康秀
Authors alternative :Higoshi, Hironobu
Moriyama, Takahiro
Moromi, Yasuhide
Issue Date :29-Nov-1980
Abstract :著者らは, これまで本県における生乳の細菌学的品質を検査してきたが, 今回はその一環として, 乳用牛群改良推進事業に参加している酪農家の乳牛から得られた生乳について, RTを行なうとともに総菌数および細胞数を測定し, さらにCMTによる乳房炎乳の検出を試みた。17酪農家の乳牛(6∿48頭)を対象に1∿3回検査を行ない, 得られた延べ881試料のRTでは, 0∿5級に分布したが, 大多数の試料(86.2%)は2級以下であった。総菌数では818試料(92.8%)が1ml当り400万以下であり, うち690試料は100万以下であった。乳牛の飼養規模別に, 総菌数が400万以下を示した試料の出現率をみると, 14頭以下の小規模酪農家(4戸)では79.0%, 15∿29頭の中規模酪農家(11戸)では94.8%, 30頭以上の大規模酪農家(2戸)では90.4%であった。また, 4戸の試料について季節別に400万以下の試料の出現率をみると, 初夏(5∿6月)が83.6%, 夏季(7∿9月)が78.7%, 秋季(10∿12月)が100%であった。一方, CMTで凝集の程度が十∿〓を示し, 乳房炎乳を疑わせた試料数は97(11.0%)であり, 意外に少なかった。この乳房炎乳の出現率は, 小規模酪農家で9.7%, 中規模酪農家で11.9%, 大規模酪農家で7.8%であり, ほぼ同じ出現率であった。CMTによる凝集の程度と細胞数の関係では, 凝集程度の強いほど細胞数が増加し, 十程度で平均細胞数は1ml当り50万を越えた。また, CMTの凝集程度とRTの級または総菌数との関係は, いずれも明確でなかった。
As a part of studies on bacteriological quality of raw milk in Okinawa Island, this survey was carried out with the resazurin test (RT), bacterial and udder cell enumerations and California Mastitis Test (CMT) for mastitis, on raw milk samples from cattle of dairy farmers participating in the Dairy Cattle Herd Improvement Promotion Project. Each of the cows in seventeen farms (6 to 48 cows/farm) was examined on three occasions, with a total of 881 milk samples. By RT, the samples ranged from classes 0 to 5,a great majority (759 samples, or 86.2%) being found to be of class 2 or lower. The direct microscopic bacterial count was less than 4,000,000/ml in 818 samples (92.8%), of which 690 showed values of less than 1,000,000/ml. Seventy-nine per cent of the samples studied from small-size farms (4) maintaining 14 or less numbers of cows showed direct microscopic bacterial counts of less than 4,000,000/ml. Corresponding rates of the samples from middle-size farms (11) with 15 to 29 cows and those from large-size farms (2) with 30 or more cows were 94.8% and 90.4%, respectively. Screening by CMT revealed an unexpectedly low incidence (97 samples, or 11.0%) of suspected mastitis, showing one-to four-plus reaction. The incidence was 9.7% in the group of small-size farms, 11.9% in the group of middle-size farms and 7.8% in that of large-size farms, respectively. As for interrelation of the CMT and the number of cells present in milk, the udder cell count increased progressively with increasing intensity of agglutination in the CMT; samples giving a one-plus reactions showed udder cell counts of more than 500,000/ml. There was no obvious correlation between the degree of agglutination in the CMT and the class in the RT or the direct microscopic bacterial count.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/4112
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.27 p.315 -322
Appears in Collections:No.27 (1980/11)

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