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No.25 (1978/12) >

Title :第 2 報 本病の発生圃場から分離された Pythiaceae 菌(パインアップル root rot wilt に関する研究)(農学科)
Title alternative :2. Pythiaceae isolated from the diseased pineapple in the Okinawa Islands (Studies on the root rot wilt of the pineapple)(Department of Agriculture)
Authors :田盛, 正雄
大嶺, 和子
Authors alternative :TaMori, Masao
Omine, Kazuko
Issue Date :1-Dec-1978
Abstract :萎ちょう病の発生圃場から, Pythiaceae菌の分離を試み, 主な菌株をパインアップルの根に接種, 根の症状を観察し, 病原性について検討した。Phytophthora cinnamomiは国頭を除く, 大宜味, 東, 今帰仁, 本部, 名護, 宜野座, 石川, 石垣, 西表と, ほとんどの圃場から分離された。Phytophthora nicotianae var. parasiticaは本部, 名護, 宜野座, 石川, 石垣, 西表から分離された。Pythium sp. (A), Pythium sp. (B)はずれも大部分の圃場から分離された。菌の分離には, 採取した根を直接用いての静置分離法と, パインアップル葉を用いてのtrap法を平行して行なった。そして, Pythiaceaeの分離割合を比較したところ, パインアップル葉を用いてのtrap法で多く分離された。Phytophthora cinnamomi, Phytophthora nicotianae var. parasitica, Pythium sp. (A), Pythium sp. (B), Fusarium sp., Ceratocystis paradoxaの6菌株を根に接種した。Phytophthora cinnamomiおよびPhytophthora nicotianae var. parasiticaを接種した根はすべて発病しており, ひどい根腐れを生じた。Pythium sp. (A)を接種した根は発病根率17∿62%で幅広い値を示したが, 発病程度はいずれも軽かった。Pythium sp. (B)を接種した根で発病したものは少なく, それらの発病程度はきわめて軽かった。
The present paper report the result of isolation test of Pythiaceae from the pineapple fields in the Okinawa Islands. Phytophthora cinnamomi was isolated from almost all fields, i. e. Ogimi, Higashi, Nakijin, Motobu, Nago, Ginoza, Ishikawa, Ishigaki and Iriomote. Phytophtohora nicotianae var. parasitica was isolated from Motobu, Nago, Ginoza, Ishikawa, Ishigaki and Iriomote. Both Phythium sp. (A) and Phythium sp. (B) were isolated from almost all fields. The two methods were applied on the isolation : (1) Take pieces from the diseased root directly. (2) Trap with young pineapple leaf and take the pieces as above. These were cultivated in V-8 juice agar medium. As compared with the isolation ratio of the Phythiaceae, this fungi were mostly isolated by means of trap method. For certify pathogenicity, six species of the isolated fungi were inoculated to the pineapple roots. These six species are as follows : Phytophthora cinnamomi, Phytophthora nicotianae var. parasitica, Phythium sp.(A), Phythium sp.(B), Fusarium sp., Ceratocystis paradoxa. As regards Phytophthora cinnamomi and Phytophthora nicotianae var. parasitica, inoculated roots were all infected and occured severe root decay. Rate of infected roots of Phthium sp. (A) had wide value (17-62%), but severity had all slight. On the Phthium sp.(B), infected roots had less and there severity had quite slight.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/4188
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.25 p.105 -114
Appears in Collections:No.25 (1978/12)

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