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No.24 (1977/12) >

 
Title :レサズリンテストを中心とする生乳の細菌学的検査(畜産学科)
Title alternative :Bacteriological examination of raw milk particular reference to resazurin test(Department of Animal Husbandry)
Authors :日越, 博信
大城, 盛徳
赤嶺, 辰雄
田港, 朝明
Authors alternative :Higoshi, Hironobu
Oshiro, Seitoku
Akamine, Tatsuo
Taminato, Tomoaki
Issue Date :1-Dec-1977
Abstract :著者らは, 沖縄における生乳の細菌による汚染状況を知る目的で, まず本島南部のミルカー使用酪農家15戸を選び, それらの工場持込み生乳について, 夏季(1976年7∿8月)と秋季(同年11∿12月)に調査を行なった。1 夏季60試料, 秋季60試料のRTでは1∿5級に分布した。しかし, 夏季試料では3級以上が多かったのに対し, 秋季試料では2級以下が多かった。2 総菌数では, 夏季の全試料が100万/ml以上であり, 食品衛生法の規格(400万/ml以下)に合格したのは20%のみで, 秋季試料でも58%が合格したにすぎなかった。一方, 1,001万を越えた試料が, 夏季では過半数を占め, 秋季では30%あった。3 生菌数は, 20°および35℃培養では夏季10^5∿10^7/ml, 秋季10^4∿10^7,5℃培養では夏季10^3∿10^6,秋季10^1∿10^5の範囲にあった。秋季試料の生菌数は, 夏季試料のそれに比べて, 培養温度に関係なく少なかった。4 大腸菌群数は, 夏季10^3∿10^6/ml, 秋季10^1∿10^5の範囲にあり, 秋季において菌数の少ない試料が多かった。5 RTの級と総菌数あるいは生菌数との関係では, 級が高くなるほどいずれの菌数も増加した。なお, RTで1級の全試料と2級の大多数は, 総菌数400万以下であった。6 今回の調査対象酪農家のうち3戸は, バルククーラーを使用していた。これら酪農家の生乳では, 他酪農家の生乳より総菌数, 20°および35℃培養菌数が少なく, 5℃培養菌数が多くなる傾向を示した。
For the purporse of finding the status of bacterial contamination of raw milk in Okinawa, Japan, the authors made bacteriological examinations of the raw milk to be delivered to a milk processing plant by 15 selected milker-using farms located in southern Okinawa Island in summer (July-August, 1976) and autumn (November-December, 1976). 1. The resazurin tests on 60 summer and 60 autumn samples were distributed in classes 1 to 5. However, the greater part of the tests on the summer samples was distributed in class 3 and higher classes, while the greater part of the tests on the autumn samples was distributed to not higher than class 2. 2. The direct microscopic counts (individual count) of all the summer samples were not less than 1,000,000/ml, only 20% of the samples meeting the standard of the Japanese Food Sanitation Law (not more than 4,000,000/ml), and even of the autumn samples, only 58% met the standard. On the other hand, more than half of the summer samples exceeded the direct microscopic count of 10,000,000/ml, and 30% of the autumn samples exceeded the count. 3. The viable bacterial counts of the summer samples by incubation at 20℃ for 4 days and 35℃ for 2 days were (10)^5-(10)^7/ml, and those of the autumn samples, (10)^4-(10)^7/ml. The psychrotrophic counts of the summer samples by incubation at 5℃ for 10 days were (10)^3-(10)^6/ml, and those of the autumn samples, (10)^1-(10)^5/ml. Thus, the viable bacterial counts of the autumn samples were less, irrespective of the incubation temperature, than those of the summer samples. 4. The coliform counts of the summer samples were (10)^3-(10)^6/ml, and those of the autumn samples, (10)^1-(10)^5/ml, thus, the autumn samples included more samples with less coliform counts. 5. There was such a relationship between the resazurin tests and the direct microscopic or viable bacterial counts that the higher the class, the greater was the bacterial count. All the samples of class 1 by the resazurin test and the majority of the samples of class 2 by the same test were below the direct microscopic count of 4,000,000/ml. 6. Of the farms selected as the subjects of the present study, 3 used the Bulk cooler. The raw milk samples from these farms were less in direct microscopic count, viable bacterial count at 20℃ and that 35℃ than the samples from the other farms, while the former tended to be greater in psychrotrophic count.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/4254
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.24 p.487 -496
Appears in Collections:No.24 (1977/12)

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