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No.23 (1976/12) >

Title :相互転座分析法による稲の連鎖分析(農学科)
Title alternative :Linkage analysis of rice plant, by the use of reciprocal translocation lines (Department of Agriculture)
Authors :佐藤, 茂俊
Authors alternative :Sato, Shigetoshi
Issue Date :1-Dec-1976
Abstract :長尾・高橋の連鎖群に細胞学的基礎を与えるために, 相互転座系統を用いて遺伝子と転座点との連鎖関係を調べた。その結果を摘録すると以下の如くである。1)第I連鎖群 : 本連鎖群には新たに第VII連鎖群のfs及びUrが加わり, 第6染色体と関連のあった遺伝子と転座点の配列順列はwx-RT6-11-C-RT6-10-fs-(RT6-8)-Cl-RT5-6-RT6-7-RT3-6-Urとなった。2)第II連鎖群 : 第11染色体と対応関係にあり, 遺伝子と転座点とはRT3-11b-d_2-RT3-11a-Pl-lg-RT6-11-RT1-11なる配列となった。3)第III連鎖群 : A, Rd及びPnの他に新たに第IX連鎖群のriがそれぞれ第3染色体に座乗し, それらは(RT3-11b)-RT1-3b-RT3-12-A-Rd-RT3-11c-RT1-3a-RT3-4b-RT3-6-Pn-riなる配列であった。4)第IV連鎖群 : 第10染色体に相当し, 遺伝子と転座点とは(RT10-11)-RT6-10-d_6-g-RT9-10a-RT8-10-Rc-RT1-10なる座位関係にあった。5)第V連鎖群 : 本連鎖群の遺伝子であるI-Bfは第1,第3,第4,第6,第8及び第12染色体の内, いずれか1本に対応していた。特に第8あるいは第12染色体の遺伝子である可能性が高かった。6)第VI連鎖群 : 第2染色体に対応し, RT1-2-RT2-3-gw-d_1-gh-RT2-5なる転座点と遺伝子との位置関係が推定された。7)第VII連鎖群 : Dnは第1染色体に座乗していた。上述の如く, fs及びUrが第I連鎖群に移行し, その代りに第IX連鎖群のnlが本染色体上にあった。Dn及びnlと転座点との配列は, Dn-RT1-11-RT1-2-RT1-3b-RT1-10-RT1-3a-nlと推定される。8)第VIII連鎖群 : laは第9染色体にあることが認められた。9)第IX連鎖群 : 前述の如く, 本群の遺伝子riは第III連鎖群に, またnlは第VII連鎖群にそれぞれ移すべきものと推定された。10)第X連鎖群 : bl_1は第8染色体にあった。11)第XI連鎖群 : bcは第5染色体に座乗していたが, d_8は第11染色体(第II連鎖群)の遺伝子となった。12)第XII連鎖群 : Hg及びglを用いてそれぞれ6及び17種の転座点との関係を調査したが, 未だ明らかな連鎖関係は認められなかった。13)なお転座点を介した場合, 2遺伝子間の組換価は正常な染色体構成における両遺伝子間の組換価より減少することが認められた。
NAGAO and TAKAHASHI first constructed twelve linkage groups corresponding to the chromosome number of a haploid plant in Japanese rice, with 37 kinds of gene loci containing anthocyanin coloration, morphological and physiological characters. Following linkage studies have been carried out based on their construction, and the results obtained were summarized by TAKAHASHI and KINOSHITA. One of important phases of linkage studies left is the relations between the twelve linkage groups and corresponding chromosomes. The linkages between established interchanged points of reciprocal translocations and marker genes have been useful. NISHIMURA produced 29 kinds of translocation lines (RT-line) from which each of twelve chromosomes can be analyzed, and IWATA and SATO et. al added some from semisteriles caused by the irradiation of atomic bomb and gamma-ray, respectively. Using these stocks (Table 1), the present author attempted to make clear relationships between linkage groups and corresponding chromosomes. The results obtained are summarized as follows. i) The first linkage group : This group corresponded to the sixth chromosome. The two genes, fs and Ur which belonged to the seventh linkage group formerly, were assignd to this group according to the linkage relationships to the interchanged points containing this chromosome. The arrangement was estimated as wx-RT6-11-C-RT6-10-fs-(RT6-8)-Cl-RT5-6-RT6-7-RT3-6-Ur(Fig.1). ii) The second linkage group : This group corresponded to the eleventh chromosome and in the order of RT3-11b-d_2-RT3-11a-P1-1g-RT6-11-RT1-11(Fig. 2). iii) The third linkage group : Three genes, A, Rd and Pn of this group had relations to the third chromosome. Besides them, ri of the ninth linkage group indicated linkage relationships with RT3-4b and RT3-6.
The order of (RT3-11b)-RT1-3b-RT3-12-A-Rd-(RT3-11c)-RT1-3a-RT3-4b-RT3-6-Pn-ri was estimated (Fig. 3). iv) The fourth linkage group : It was estimated that three genes, d_6,g and Rc of this group belonged to the tenth chromosome, and the order of genes and interchanged points was (RT10-11)-RT6-10-d_6-g-RT9-10a-RT8-10-Rc-RT1-10(Fig. 4). v) The fifth linkage group : I-Bf had relations with 4 kinds of interchanged points, T1-4b, RT3-6,RT8-12a and RT8-12b. Therefore, it might be carried on one of the six chromosomes. From the significance of chi-square values for linkage, it was considered that the gene placed on one of two chromosomes; the eighth and the twelfth. vi) The sixth linkage group : This group had a relation with the second chromosome and the order of RT1-2-RT2-3-gw-dl-gh-RT2-5 was estimated (Fig. 5). vii) The seventh linkage group : As mentioned above, fs and Ur shifted to the first linkage group. Only the gene, Dn belonged to the first chromosome. Furthermore, the gene, nl to have belonged to the ninth group, joined to this group in the order of Dn-RT1-11-RT1-2-RT1-3b-RT1-10-RT-3a-nl(Fig. 6). viii) The eighth linkage group : The gene, la belonged to the ninth chromosome. ix) The ninth linkage group : As mentioned above, ri and nl moved to the third (the third group) and the first chromosome (the seventh group), respectively. Marker genes of this group have not yet been found out. A new linkage group must be established. x) The tenth linkage group : The gene, bl_1 had a linkage relation with interchanged points involving the eighth chromosome. xi) The eleventh linkage group : The gene, bc was carried on the fifth chromosome. The gene, d_8 replaced on the eleventh chromosome which corresponded to the second group. xii) The twelfth linkage group : Two genes, gl and Hg did not indicate any linkage relationships with interchanged points examined. xiii) The discrepancy between the present author's results and those of IWATA and OMURA on the third linkage group must be reexamined. xiv) In the case where a interchanged point (RT6-11) was existent between C and wx, a recombination value between them was greatly reduced than that in normal plant (Table 6).
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/4290
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.23 p.73 -104
Appears in Collections:No.23 (1976/12)

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