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The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus >
No.22 (1975/12) >

 
Title :2,4 -ジニトロフェニルヒドラジンを用いるタウリンーピルビン酸トランスアミナーゼの活性測定法(農芸化学科)
Title alternative :An assay method of taurine-pyruvate transaminase activity with 2,4- dinitrophenylhydrazine (Department of Agricultural Chemistry)
Authors :当山, 清善
安田, 正昭
左右田, 健次
Authors alternative :Toyama, Seizen
Yasuda, Masaaki
Soda, Kenji
Issue Date :1-Dec-1975
Abstract :タウリンーピルビン酸トランスアミナーゼ活性を2,4-ジニトロフェニルヒドラゾンを用いて測定する方法を検討し, 次の結果を得た。酵素反応混液のピルビン酸とスルホアセトアルデヒドを2,4-ジニトロフェニルヒドラゾンとした後トルオールで抽出すると, ピルビン酸のヒドラゾンはトルオール層に抽出され, スルホアセトアルデヒドのヒドラゾンは水層に残留する。水層中のスルホアセトアルデヒドヒドラゾンはアルカリ性で発色させると435mμに吸収極大を有する。酵素活性は本吸収極大の吸光度を定量することによって測定し得る。
This communication describes an assay method of taurine : pyruvate transaminase with 2,4- dinitrophenylhydrazine. The results obtained were as follows; Pyruvic acid and sulfoacetaldehyde in the enzyme reaction mixture were allowed to react with 2,4- dinitrophenylhydrazine, after which the hydrazones formed were separated from each other with toluol. The pyruvic acid hydrazone was extracted to the toluol layer and the sulfoacetaldehyde hydrazone remained in the aqueous layer. The sulfoacetaldehyde hydrazone of the aqueous layer gave an intense red brown color with the mixture of NaOH and Na_2CO_3 solution, exhibiting the absorption maximum at 435mμ. The transaminase activity could be determined by measuring the absorbance at the absorption peak.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/4333
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.22 p.215 -223
Appears in Collections:No.22 (1975/12)

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