HOME    About this site    mypage    Japanese    library    university    Feedback

University of the Ryukyus Repository >
Faculty of Agriculture >
Bulletin >
The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus >
No.18 (1971/12) >

 
Title :イヌ伝染性肝炎ウイルスでトランスホームしたハムスター胎児細胞からの株化細胞の作出とその性状(畜産学科)
Title alternative :Characteristics of cell lines established from hamster embryo cells transformed by infectious canine hepatitis virus (Department of Animal Husbandry)
Authors :金城, 俊夫
Authors alternative :Kinjo, Toshio
Issue Date :1-Dec-1971
Abstract :Two cell lines, named W-16 and THW, were successfully established from the hamster embryo cells transformed by infectious canine hepatitis virus. During early passage levels, the both cell lines were cultivated with Hanks balanced salt solution with low calcium content, 0.1mM, however, after about 10 passages, they were feeded with usual Hanks BSS (1.2mM). ICH virus specific T antigen was demonstrated either in almost all cells by fluorescent antibody staining or in hamster tumors induced with these cells by complement fixation test. The cells continuously possessed their oncogenic potency for hamsters. ICH virus isolation in fused cells between the transformed hamster cells and dog kidney cells was negative. The data seemed to indicate that the viral genes, required for transformation, are a small part of total viral genes.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/4499
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.18 p.185 -198
Appears in Collections:No.18 (1971/12)

Files in This Item:

File Description SizeFormat
KJ00000162379.pdf1135KbAdobe PDFView/Open