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Title :Pseudotyping of HIV-1 with Human T-Lymphotropic Virus 1 (HTLV-1) Envelope Glycoprotein during HIV-1–HTLV-1 Coinfection Facilitates Direct HIV-1 Infection of Female Genital Epithelial Cells : Implications for Sexual Transmission of HIV-1
Authors :Tang, Yuyang
George, Alvin M.
Petrechko, Oksana
Nouvet, Franklin J.
Sweet, Stephanie D.
Tanaka, Yuetsu
Imbiakha, Brian S.
Jiang, Guochun
Gao, Wei
Anastos, Kathryn
Hildreth, James E.K.
Issue Date :Apr-2018
Abstract :Female genital epithelial cells cover the genital tract and provide the first line of protection against infection with sexually transmitted pathogenic viruses. These cells normally are impervious to HIV-1. We report that coinfection of cells by HIV-1 and another sexually transmitted virus, human T-lymphotropic virus 1 (HTLV-1), led to production of HIV-1 that had expanded cell tropism and was able to directly infect primary vaginal and cervical epithelial cells. HIV-1 infection of epithelial cells was blocked by neutralizing antibodies against the HTLV-1 envelope (Env) protein, indicating that the infection was mediated through HTLV-1 Env pseudotyping of HIV-1. Active replication of HIV-1 in epithelial cells was demonstrated by inhibition with anti-HIV-1 drugs. We demonstrated that HIV-1 derived from peripheral blood of HIV-1–HTLV-1-coinfected subjects could infect primary epithelial cells in an HTLV-1 Env-dependent manner. HIV-1 from subjects infected with HIV-1 alone was not able to infect epithelial cells. These results indicate that pseudotyping of HIV-1 with HTLV-1 Env can occur in vivo. Our data further reveal that active replication of both HTLV-1 and HIV-1 is required for production of pseudotyped HIV-1. Our findings indicate that pseudotyping of HIV-1 with HTLV-1 Env in coinfected cells enabled HIV-1 to directly infect nonpermissive female genital epithelial cells. This phenomenon may represent a risk factor for enhanced sexual transmission of HIV-1 in regions where virus coinfection is common.
URL :https://doi.org/10.1128/mSphere.00038-18
Type Local :雑誌掲載論文
ISSN :2379-5042
Publisher :American Society for Microbiology Journals
URI :http://hdl.handle.net/20.500.12000/45973
Citation :mSphere Vol.3 no.2
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Medicine)

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