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Title :Sequence Analysis of a Total of Three Megabases of DNA in Two Regions of Chromosome 8p
Authors :Isomura, Minoru
Ikegawa, Shiro
Kinjo, Takao
Takeuchi, Kumiko
Yamane-Tanaka, Yuka
Kitami, Kikuko
Nakamura, Yusuke
Issue Date :1-Dec-1999
Abstract :Large-scale sequencing of genomic regions and in silico gene trapping together represent a highly efficient and powerful approach for identifying novel genes. We performed megabase-level sequence analyses of two genomic regions on human chromosome 8p (8p11.2 and 8p22.→p21.3), after covering those segments with sequence-ready contigs composed of 74 cosmids, 14 BACs, and three PAC clones. We determined continuous nucleotide sequences of 1,856,753 bases on 8p11.2 and 1,210,381 bases on 8p22→p21.3 by combining the shotgun and primer-walking methods. In silico gene trapping identified four novel genes in the 8p11.2 region and, in the 8p22→p21.3 region, six known genes ( PRLTS, PCM1, MTMR7, HCAT2, HFREP-1 and PHP ) and three novel genes. The distribution of Alu and LINE1 repetitive elements and the densities of predicted exons were different in each region, and Alu -rich portions contained more exonic sequences than LINE1-rich areas.
URL :https://doi.org/10.1093/dnares/6.6.387
Type Local :雑誌掲載論文
ISSN :1340-2838
Publisher :Oxford University Press
URI :http://hdl.handle.net/20.500.12000/46345
Citation :DNA Research Vol.6 no.6 p.387 -400
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Medicine)

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