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Title :GABA/glycine signaling during degeneration and regeneration of mouse hypoglossal nerves
Authors :Tatetsu, Masaharu
Kim, Jeongtae
Kina, Shinichiro
Sunakawa, Hajime
Takayama, Chitoshi
Issue Date :29-Mar-2012
Abstract :In the adult central nervous system (CNS), GABA and glycine (Gly) are predominant inhibitory neurotransmitters, negatively regulating glutamatergic transmission. In the immature CNS, on the other hand, they act as trophic factors, mediating morphogenesis. In the present study, to investigate their involvement in axonal regeneration, we morphologically examined changes in their signaling in mouse hypoglossal nuclei during degeneration and regeneration of hypoglossal nerves. We found that (1) expression and localization of presynaptic elements were not changed, (2) localization of gephyrin, which anchors GABA and Gly receptors, was spread on the surface of motor neuron cell bodies and dendrites, (3) KCC2-expression markedly decreased, (4) choline acetyltransferase, which mediates acetylcholine-synthesis, immediately disappeared from the motor neurons, and (5) the synaptic cleft of both excitatory and inhibitory synapses became irregularly wider, in the hypoglossal nuclei of the sutured side after the operation. These changes gradually normalized during regeneration. These results suggested that synthesis of acetylcholine may be stopped in the motor neuron after axotomy. GABA/Gly may be normally released from presynaptic terminals, be spilled over the original synaptic cleft, be diffused into the neighboring space, bind to extrasynaptically localized receptors, and mediate depolarization of the membrane potential of motor neurons during degeneration and regeneration. Furthermore, it was suggested that GABA/Gly signaling in postsynaptic motor neurons went back to being immature after axotomy, and may play an important role in axonal regeneration.
URL :https://doi.org/10.1016/j.brainres.2012.01.048
Type Local :雑誌掲載論文
ISSN :0006-8993
Publisher :Elsevier
URI :http://hdl.handle.net/20.500.12000/46546
Citation :Brain Research Vol.1446 p.22 -33
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Medicine)

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