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Title :Comprehensive genetic diagnosis of Japanese patients with severe proteinuria
Authors :Nagano, China
Yamamura, Tomohiko
Horinouchi, Tomoko
Aoto, Yuya
Ishiko, Shinya
Sakakibara, Nana
Shima, Yuko
Nakanishi, Koichi
Nagase, Hiroaki
Iijima, Kazumoto
Nozu, Kandai
Issue Date :14-Jan-2020
Abstract :Numerous disease-causing gene mutations have been identified in proteinuric diseases, such as nephrotic syndrome and glomerulosclerosis. This report describes the results of comprehensive genetic diagnosis of Japanese patients with severe proteinuria. In addition, the report describes the clinical characteristics of patients with monogenic disease-causing mutations. We conducted comprehensive gene screening of patients who had either congenital nephrotic syndrome, infantile nephrotic syndrome, steroid-resistant nephrotic syndrome, or focal segmental glomerular sclerosis. Using targeted next-generation sequencing, 60 podocyte-related genes were screened in 230 unrelated patients with proteinuria. A retrospective review of clinical data was conducted for these patients. We detected monogenic disease-causing mutations in 30% (69 of 230) of patients among 19 of the screened genes. Common genes with disease-causing mutations were WT1 (25%), NPHS1 (12%), INF2 (12%), TRPC6 (10%), and LAMB2 (9%). With various immunosuppressive or renoprotective therapies, remission of proteinuria in patients with unknown causative mutations was observed in 26% of patients, whereas only 5% of patients with monogenic disease-causing mutations exhibited complete remission. We assessed the genetic backgrounds of Japanese patients with severe proteinuria. The proportion of patients with gene defects was similar to that of other reports, but the disease-causing gene mutation frequency was considerably different.
URL :https://doi.org/10.1038/s41598-019-57149-5
Type Local :雑誌掲載論文
ISSN :2045-2322
Publisher :Springer Nature
URI :http://hdl.handle.net/20.500.12000/46988
Citation :Scientific Reports Vol.10 no.1
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Medicine)

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