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Title :Inhibitory effects of pine nodule extract and its component, SJ-2, on acetylcholine-induced catecholamine secretion and synthesis in bovine adrenal medullary cells
Authors :Li, Xiaojia
Horishita, Takafumi
Toyohira, Yumiko
Shao, Hui
Bai, Jie
Bo, Haixia
Song, Xinbo
Ishikane, Shin
Yoshinaga, Yukari
Satoh, Noriaki
Tsutsui, Masato
Yanagihara, Nobuyuki
Issue Date :Apr-2017
Abstract :Extract of pine nodules (matsufushi) formed by bark proliferation on the surface of trees of Pinus tabulaeformis or Pinus massoniana has been used as an analgesic for joint pain, rheumatism, neuralgia, dysmenorrhea and other complaints in Chinese traditional medicine. Here we report the effects of matsufushi extract and its components on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. We found that matsufushi extract (0.0003–0.005%) and its component, SJ-2 (5-hydroxy-3-methoxy-trans-stilbene) (0.3–100 μM), but not the other three, concentration-dependently inhibited catecholamine secretion induced by acetylcholine, a physiological secretagogue. Matsufushi extract (0.0003–0.005%) and SJ-2 (0.3–100 μM) also inhibited ^<45>Ca^<2+> influx induced by acetylcholine in a concentration-dependent manner, similar to its effect on catecholamine secretion. They also suppressed 14C-catecholamine synthesis and tyrosine hydroxylase activity induced by acetylcholine. In Xenopus oocytes expressing α3β4 nicotinic acetylcholine receptors, matsufushi extract (0.00003–0.001%) and SJ-2 (1–100 μM) directly inhibited the current evoked by acetylcholine. The present findings suggest that SJ-2, as well as matsufushi extract, inhibits acetylcholine-induced catecholamine secretion and synthesis by suppression of nicotinic acetylcholine receptor-ion channels in bovine adrenal medullary cells.
URL :https://doi.org/10.1016/j.jphs.2017.03.006
Type Local :雑誌掲載論文
ISSN :1347-8613
Publisher :Elsevier
URI :http://hdl.handle.net/20.500.12000/47186
Citation :Journal of Pharmacological Sciences Vol.133 no.4 p.268 -275
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Medicine)

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