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Title :イネ雄性不稔細胞質の分類 (2)
Title alternative :Classification of Male Sterile Cytoplasms in Rice
Authors :本村, 恵二
松園, 達也
石嶺, 行男
赤嶺, 光
Authors alternative :Motomura, Keiji
Matsuzono, Tatsuya
Ishimine, Yukio
Akamine, Hikaru
Issue Date :1-Jun-1994
Abstract :イネにおける雄性不稔細胞質を分類するために, 野生稲由来の12細胞質雄性不稔系統RT2S, RT4S, RT7S, RT18S, RT41S, RT80S, RT81S, RT124S, RT132S, RT134S, RT142SおよびRT153Sが被検定親として供試された.また, 検定親として5稔性回復系統RT61F, BTF, RT98F, RT100FおよびRT102Fが用いられた.いずれも連続戻し交雑法により育成された台中65号の同質遺伝子系統であり, RT61FおよびBTFは同一の稔性回復遺伝子をもつが, 他の稔性回復系統はそれぞれ異なる1対の遺伝子をもっている.これらの間で交雑を行い, F_1の花粉および種子稔性を調査して細胞質の分類を行った.ただし, 花粉の稔・不稔の判定は一部を除いて困難であったため, 分類には主として種子稔性が用いられた.種子稔性は不稔(10%以下), 低稔(10~50%), 高稔(50~90%), 正常稔(90%以上)の4段階に分けられた.その結果次のように分類できた.(1)RT18SおよびRT153Sはすべての稔性回復系統とのF_1で正常稔を示した.(2)RT4Sはすべての交雑で完全不稔を示した.(3)RT2S, RT7S, RT41S, RT132SおよびRT134Sは, RT61FおよびBTFとの交雑で不稔, RT98F, RT100FおよびRT102Fとの交雑で高稔を示した.(4)RT80SおよびRT124Sの2系統はRT61FおよびBTFとの交雑で完全不稔, RT98Fとの交雑で高稔, RT100Fとの交雑で低稔, RT102Fとの交雑で完全不稔に近い稔性を示した.(5)RT81SおよびRT142Sの2系統はRT61FおよびBTFとの交雑でともに完全不稔を示し, RT98Fとの交雑で低稔, RT100FおよびRT102Fとの交雑で完全不稔に近い稔性を示した.以上は種子稔性による分類であったが, 花粉稔性ではRT18SおよびRT153SのF_1は配偶体型の細胞質に特有の半稔性を示したのに対してその他の系統のF_1は全て種子稔性に関係なく完全稔を示した.
In order to classify male sterile cytoplasms in rice, twelve cytoplasmic male sterile lines (RT2S, RT4S, RT7S, RT18S, RT41S, RT80S, RT81S, RT124S, RT132S, RT134S, RT142S, and RT153S) were crossed with five restoring testers (RT61F, BTF, RT98F, RT100F and RT102F). These lines consisted of Taichung 65's isogenic lines developed through repeated back-crossing of Taichung 65 to wild rice, Oryza ruffipogon and O. breviligulata (only RT153S). The pollen and seed fertilitiy of the F_1 progenies which were derived from crosses between cytoplasmic male sterile lines and restoring testers was evaluated. Based on the results obtained, the cytoplasms of the male sterile lines were classified. The pollen of the F_1s of RT18S and RT153S which was semi-fertile (50%) was characteristic of a gametophytic cytoplasm. On the other hand, the pollen fertility of all the lines except for these two lines was complete in the cace of hybridization with all the testers, regardless of the seed fertility. Further classification is required for the utilization of seed fertility. RT18S and RT153S showed a normal seed fertility (more than 90%) in the F_1 for all the testers. RT4S showed a complete sterility for the seeds of F_1 progenies in the crosses with all the testers. The F_1s of RT2S, RT7S, RT41S, RT132S and RT134S displayed a seed sterility (less than 1%) in the crosses with RT61F and BTF, and a higher seed fertility (70~80%) in the crosses with RT98F, RT100F and RT102F. The RT80S and RT124S lines produced seed-sterile F_1 plants in the crosses with RT61F and BTF, a higher fertility in the crosses with RT98F, a lower fertility in the crosses with RT100F, and near-complete sterility in the crosses with RT102F. The RT81S and RT142S lines produced complete sterile seeds in the crosses with RT61F and BTF, but showed a lower seed fertility in the crosses with RT98F and near-complete seed sterility in the crosses with RT100F and RT102F. The cytoplasms of the twelve cytoplasmic male sterile lines were classified into the following groups : (1) RT18S and RT153S (2) RT4S (3) RT2S, RT7S, RT41S, RT132S and RT134S (4) RT80S and RT124S (5) RT81S and RT142S.
Type Local :雑誌掲載論文
ISSN :0021-5260
Publisher :日本熱帯農業学会
URI :http://hdl.handle.net/20.500.12000/4908
Citation :熱帯農業 = Japanese Journal of Tropical Agriculture Vol.38 no.2 p.93 -97
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Agriculture)

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