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The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus >
No.53 (2006/12) >

Title :Detection of Citrus Huanglongbing using an Iodo-starch Reaction
Title alternative :ヨウ素デンプン反応を利用したカンキツグリーニング病の検出
Authors :Taba, Satoshi
Nasu, Kanami
Takaesu, Kazuko
Ooshiro, Atsushi
Moromizato, Zen-ichi
Authors alternative :田場, 聡
那須, 奏美
高江洲, 和子
大城, 篤
諸見里, 善一
Issue Date :Dec-2006
Abstract :A rapid, low cost method for the detection of physiological changes associated with the causal agent of huanglongbing (greening) in citrus was developed. A leaf of citrus was cut into 5x5 mm pieces, crushed and softened with hot water for 10 min. A 100 μl sample of sap containing the infectious agent placed in a micro plate with 40 μl 5 mM iodine solution turned purple as a result of the iodo-starch reaction. Leaf samples from trees with deficiency symptoms gave a reaction ranging from light blue to blue, and healthy tree samples became yellow or brown, a result that distinguished diseased trees from healthy ones with the naked eye. Moreover, diseased samples had 20 times the optical absorbance as healthy ones at 540 nm. A comparison of PCR results with the iodo-starch assay resulted in 75% agreement for leaf samples, and 95% for trees.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/607
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.53 p.19 -24
Appears in Collections:No.53 (2006/12)

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