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Title :Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules
Authors :Teruya, Hiromitsu
Tomita, Mariko
Senba, Masachika
Ishikawa, Chie
Tamayose, Maki
Miyazato, Akiko
Yara, Satomi
Tanaka, Yuetsu
Iwakura, Yoichiro
Fujita, Jiro
Mori, Naoki
Issue Date :22-Sep-2008
Abstract :[Background] Human T-cell leukemia virus type I (HTLV-I) is associated with pulmonary diseases, characterized by bronchoalveolar lymphocytosis, which correlates with HTLV-I proviral DNA in carriers. HTLV-I Tax seems to be involved in the development of such pulmonary diseases through the local production of inflammatory cytokines and chemokines in T cells. However, little is known about induction of these genes by HTLV-I infection in lung epithelial cells.
[Results] We tested infection of lung epithelial cells by HTLV-I by coculture studies in which A549 alveolar and NCI-H292 tracheal epithelial cell lines were cocultured with MT-2, an HTLV-I-infected T-cell line. Changes in the expression of several cellular genes were assessed by reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay and flow cytometry. Coculture with MT-2 cells resulted in infection of lung epithelial cells as confirmed by detection of proviral DNA, HTLV-I Tax expression and HTLV-I p19 in the latter cells. Infection was associated with induction of mRNA expression of various cytokines, chemokines and cell adhesion molecule. NF-κB and AP-1 were also activated in HTLV-I-infected lung epithelial cells. In vivo studies showed Tax protein in lung epithelial cells of mice bearing Tax and patients with HTLV-I-related pulmonary diseases.
[Conclusion] Our results suggest that HTLV-I infects lung epithelial cells, with subsequent production of cytokines, chemokines and cell adhesion molecules through induction of NF-κB and AP-1. These changes can contribute to the clinical features of HTLV-I-related pulmonary diseases.
URL :http://www.retrovirology.com/content/5/1/86
Type Local :雑誌掲載論文
ISSN :1742-4690
Publisher :BioMed Central Ltd.
URI :http://hdl.handle.net/20.500.12000/7347
Citation :Retrovirology Vol.5 p.86
Appears in Collections:Peer-reviewed Journal Articles (Faculty of Medicine)

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