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No.55 (2008/12) >

 
Title :沖縄島産マングースを用いたペスト抗体産生実験およびペスト抗体保有調査
Title alternative :Experiment of the antibody response and serological survey of plague with small Asian mongoose captured on Okinawajima island.
Authors :石橋, 治
西島, 拓
角田, かおり
須藤, 健二
山下, 勝弘
小倉, 剛
砂川, 勝徳
仲田, 正
Authors alternative :Ishibashi, Osamu
Nishijima, Taku
Sumida, Kaori
Sudo, Kenji
Yamashita, Katsuhiro
Ogura, Go
Sunagawa, Katsunori
Nakada, Tadashi
Issue Date :Dec-2008
Abstract :We tried to the immunological experiment of antibody response with small Asian mongoose (Hepestes javanicus) captured on Okinawajima island, which inoculated plague (Yersinia pestis) vaccine, and then carried out the serological test measuring anti-plague antibody titer by IgG-ELISA. Three groups (each of four individuals) of mongoose inoculated by the different ways which in subcutaneous on their back, intradermal on their back, or in the crural muscle, respectively, and another three groups of mongoose inoculated with undiluted plague vaccine, diluted one at 1:10, or 1:10 with freund's adjuvant, respectively were compared. As the result, the group of mongoose inoculated in intradermal was found to increase of anti-plague antibody titer, and inoculated in diluted at 1:10 with adjuvant was found to higbIy increase of anti-plague antibody titer. The cutoff value (the average OD value + 3×standard deviation) calculated from negative control sera was 0.46, and the ratio of individual over the cutoff value was 5.6% (13 out of 231) by IgG-ELISA.
ジャワマングースに対しペストF1抗原を負荷する免疫実験を行い,ペスト抗体産生の推移をIgG-ELISA法により,陽性および陰性の判断基準を求めた.その結果,免疫負荷は皮内接種により明確な抗体価の上昇を確認し,また,カットオフ値を0.46とした.次に抗-フェレットIgGを用いて,ペスト特異タンパク質F1を抗原として沖縄島のジャワマングースについて,2002年11月から2004年10月に捕獲された231頭における抗体保有調査を行った.カットオフ値を超える個体は5.2%であった.
Type Local :紀要論文
ISSN :0370-4246
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/9907
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.55 p.1 -5
Appears in Collections:No.55 (2008/12)

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