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No.55 (2008/12) >

Title :沖縄島産マングースにおけるELISA法を用いた血清診断のための二次抗体の選定
Title alternative :Selection and test of the serodiagnostic test with IgG-ELISA method in the small Asian mongoose.
Authors :石橋, 治
角田, かおり
西島, 拓
飯塚, 信二
須藤, 健二
山下, 勝弘
小倉, 剛
砂川, 勝徳
仲田, 正
Authors alternative :Ishibashi, Osamu
Sumida, Kaori
Nishijima, Taku
Iizuka, Shinji
Sudo, Kenji
Yamashita, Katsuhiro
Ogura, Go
Sunagawa, Katsunori
Nakada, Tadashi
Issue Date :Dec-2008
Abstract :The small Asian mongoose (Herpestes javanicus) was introduced from India in 1910 to control rat and habu snake (Protobothrops flavoviridis) populations on Okinawajima island. It has been clarified that the mongoose possesses Leptospira and Salmonella. On human beast common infectious disease with these situations or the possibility in which the mongoose propagates, it is very important to investigate the infection status in prevention of epidemic. There is variously serodiagnostic method epidemiologically used at present, and it has the defect for economical efficiency and sensitivity. However, it is possible to comparatively little do in labor and time that the IgG-ELISA method is excellent for economical efficiency and both sensitivity and that it catches it in the experiment. In this experiment, secondary antibody which was optimum for doing the IgG-ELISA method using the serum of the mongoose for the mongoose was selected. Eleven kinds of secondary antibody marketed were used for the experiment. In-human, rabbit, rat, guinea pig, hamster, cat, mouse IgG, protein G, protein L that there was color reaction by the junction with IgG antibody of the mongoose in two types of anti-ferret IgG and protein A and that the hoop resists it, the reaction hardly could be accepted. However, there was the significance for OD value which became an index to the intensity of the reaction by the individual in protein A. And, the difference of the OD value between individuals of the result which the OD value is inferior to protein A showed the proportion of the change in which the OD value is fixed for the dilution degree of the secondary antibody small in anti-ferret IgG. Therefore, available and optium secondary antibody to the mongoose seems to be anti-ferret IgG.
Type Local :紀要論文
Publisher :琉球大学農学部
URI :http://hdl.handle.net/20.500.12000/9908
Citation :琉球大学農学部学術報告 = The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus no.55 p.7 -10
Appears in Collections:No.55 (2008/12)

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